Dorsoventral-mediated <i>Shh</i> induction is required for axolotl limb regeneration.

ABSTRACT: Axolotls (Ambystoma mexicanum) exhibit a remarkable ability to regenerate limbs. Classical experiments have suggested that contact between cells derived from distinct orientations-dorsal, ventral, anterior, and posterior-within the regenerating blastema is necessary for accurate limb pattern formation. However, the molecular basis for this requirement has remained largely unknown. Here, we demonstrate that both dorsal and ventral tissues are required for limb formation via induction of Shh expression, which plays a crucial role in limb patterning. Using the accessory limb model, we induced position-specific blastemas lacking cells derived from a single orientation (anterior, posterior, dorsal, or ventral). Limb patterning occurred only in blastemas containing both dorsal- and

SUBMITTER: Yamamoto S 

PROVIDER: S-EPMC12875611 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature