Schiff base deprotonation and structural changes in a mouse UV-sensitive cone visual pigment revealed by FTIR spectroscopy at 77 K.

ABSTRACT: UV-sensitive cone visual pigments are widespread among vertebrates, including birds, fish, and rodents such as mice, and play essential roles in non-primate vision. Unlike visible-light pigments, which contain a protonated retinal Schiff base and absorb at 400-700 nm, UV pigments maintain a deprotonated Schiff base, but how this state is stabilized has remained unresolved. Here, we applied low-temperature FTIR spectroscopy to the mouse UV-sensitive cone visual pigment (MUV), capturing structural changes associated with Batho intermediate formation. Spectral features characteristic of a deprotonated Schiff base were observed in both the initial and Batho states. Importantly, analysis of the Glu113 mutant demonstrated that Glu113 is protonated under these condition. Combined with the analysi

SUBMITTER: Mizuno Y 

PROVIDER: S-EPMC12875767 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature