Development and partial validation of an RT-qPCR assay for the rapid detection of spring viremia of carp virus (SVCV).

ABSTRACT: Spring viremia of carp (SVC), caused by spring viremia of carp virus (SVCV), is a highly contagious disease that poses a serious threat to cyprinid aquaculture and international trade, and it is listed as a notifiable disease by the World Organization for Animal Health (WOAH). Effective surveillance and control of SVCV rely on accurate and highly sensitive molecular diagnostic methods. However, several previously published RT-qPCR assays contain mismatches between primer/probe sequences and viral genomes, which may lead to false-negative results and reduced diagnostic reliability. In this study, a whole-genome comparison of 24 representative SVCV strains covering all four genotypes (SVCVa-d) was conducted, and a new primer-probe set (Cefas AR) targeting a highly conserved region of the L g

SUBMITTER: Zhu P 

PROVIDER: S-EPMC12875918 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature