Microtubule dynamics in adult retinal ganglion cells and dorsal root ganglion neurons.

ABSTRACT:

Introduction

While axon regeneration is very limited in the adult central nervous system (CNS) in vivo, this is not the case in the peripheral nervous system (PNS). Indeed, both CNS and PNS neurons can regenerate in vitro although to varying degrees. Given the role of microtubule stabilization in promoting regeneration, we have examined microtubule polymerization during the regeneration of two types of adult neurons in vitro, retinal ganglion cells (RGCs) from the CNS and dorsal root ganglion (DRG) neurons from the PNS.

Methods

In order to compare microtubule dynamics between these cell types, the density, polymerization rate and orientation of microtubules have been analysed during neurite regeneration in both cell types by analysing GFP-tagged Microtubule End Binding 3 (EB

SUBMITTER: Vecino E 

PROVIDER: S-EPMC12876149 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature