Valorization of Pineapple Peel Waste through Immobilized Crude Bromelain for Enhanced Feed Protein Hydrolysis.

ABSTRACT: Pineapple processing generates considerable waste with byproducts (peels, cores, crowns, and leaves) often containing high levels of bromelain, a proteolytic enzyme with promising applications in animal feed. However, the instability and low activity of crude bromelain (CBr) in aqueous form limit its industrial utilization. In this study, CBr was extracted from pineapple peel waste and directly immobilized onto a bentonite (Bt)-carboxymethylcellulose (CMC) composite without further purification. The CBr-Bt-CMC composites were prepared via ionotropic gelation, with cysteine incorporated to enhance enzymatic performance. The composite with a bromelain-to-cysteine mass ratio of 1:65 could enhance catalytic activity by over 1,000% compared to the control without cysteine. It exhibited an immob

SUBMITTER: Skulborisutsuk C 

PROVIDER: S-EPMC12878712 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature