Expanded Functional Characterization and Optimization of Protein Expression in Treponema denticola Shuttle Plasmids.

ABSTRACT: Oral spirochetes are among the small group of keystone pathogens contributing to dysregulation of periodontal tissue homeostasis, leading to breakdown of the tissue and bone supporting the teeth in periodontal disease. Of the more than 60 oral Treponema species and phylotypes, Treponema denticola is one of the few that can be grown in culture and the only one in which genetic manipulation is practicable. T. denticola is thus a model organism for studying spirochete behavior, metabolism, and interactions with other microbes and host tissues that are relevant to oral diseases. We recently demonstrated enhanced transformation efficiency using a synthetic shuttle plasmid resistant to T. denticola restriction-modification systems. Here, we report further optimization of the shuttle plasmid syst

SUBMITTER: Goetting-Minesky MP 

PROVIDER: S-EPMC12878829 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature