Profiling Allogeneic HLA-specific B-cell Responses Utilizing a 64-plex Single-HLA Reporter Cell Panel.

ABSTRACT: Identifying allogeneic HLA-specific B cells in sensitized individuals is essential for defining the cellular basis of allogeneic humoral immunity but remains technically challenging due to their low frequency. To overcome this barrier, we generated a 64-plex single-HLA reporter cell (HLA64-RC) panel that provides a cost-efficient, multiplex, high-throughput platform for screening B-cell specificity. We additionally developed a companion R package, HLA64, for automated data analysis and visualization. Integrated with a streamlined high-throughput BCR discovery workflow, this platform enables reliable identification and characterization of allogeneic HLA-specific B cells from sensitized transplant candidates. In a pilot application, thirteen HLA-specific B cells were identified, enabl

SUBMITTER: Song S 

PROVIDER: S-EPMC12879672 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature