Ontology highlight
ABSTRACT:
SUBMITTER: Humphreys DT
PROVIDER: S-EPMC1287990 | biostudies-literature | 2005 Nov
REPOSITORIES: biostudies-literature
Humphreys David T DT Westman Belinda J BJ Martin David I K DI Preiss Thomas T
Proceedings of the National Academy of Sciences of the United States of America 20051115 47
MicroRNAs (miRNAs) repress translation of target mRNAs by interaction with partially mismatched sequences in their 3' UTR. The mechanism by which they act on translation has remained largely obscure. We examined the translation of mRNAs containing four partially mismatched miRNA-binding sites in the 3' UTR in HeLa cells cotransfected with a cognate miRNA. The mRNAs were prepared by in vitro transcription and were engineered to employ different modes of translation initiation. We find that the 5' ...[more]