Development of a New Method for the Absolute Quantification of Selenoproteins in Chicken Serum by Heteroatom-Tagged Proteomics.

ABSTRACT: Selenium (Se) is an essential trace element for chickens that develops, through selenoproteins, important physiological functions. Although selenogen expression and Western blot analyses provided valuable information, there is a lack of information about the serum selenoproteome and quantitative data. In this work, we developed a new analytical method for the absolute quantification of selenoproteins and total selenometabolites in chicken serum using heteroatom-tagged proteomics, based on chromatographic separation and Se detection as a "tag" in an atomic detector. The approach was combined with bottom-up proteomics using liquid chromatography coupled to organic mass spectrometry after a tryptic digestion, and selenoprotein W was identified in chicken serum. Our results indicated that the

SUBMITTER: Callejon-Leblic B 

PROVIDER: S-EPMC12879916 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature