Identification of antimicrobial compounds in Dipsacus inermis via phytochemical profiling, in vitro assessment, and advanced computational techniques.

ABSTRACT: Antimicrobial resistance (AMR) poses a major challenge in treating infections such as pneumonia and typhoid fever, necessitating novel therapeutics. Plant-derived natural products provide a promising alternative. This study evaluated dichloromethane (DCM) and methanol (MeOH) extracts of Dipsacus inermis against six bacterial strains: Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and Enterobacter aerogenes. Antibacterial activity was assessed following standardized CLSI guidelines for both zone of inhibition (ZOI) and minimum inhibitory concentration (MIC) assays. The DCM extract demonstrated superior activity, with ZOI values of 17.87 ± 0.23 mm (S. aureus and E. aerogenes) and 16.83 ± 0.29 mm (S. typhi), and MICs of 1.562 mg/mL (B. su

SUBMITTER: Drakhshaan 

PROVIDER: S-EPMC12880709 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature