<i>Streptococcus pneumoniae</i> colonization modulates human nasal epithelial responses to respiratory syncytial virus infection.

ABSTRACT: Respiratory syncytial virus (RSV) is a major cause of morbidity and mortality in infants globally. Specific nasal bacterial genera are differentially associated with RSV severity in infants: Haemophilus and Streptococcus with more severe disease and Dolosigranulum with healthy controls or milder outcomes. We hypothesized these differential bacterial effects begin at the epithelial level. Therefore, we established human nasal epithelial organoids differentiated at air-liquid interface (HNO-ALI) as a model system to assess effects of individual nasal microbionts on the epithelial response to subsequent RSV infection and of RSV on those microbionts. Infant-derived HNO-ALI were monocolonized with either Streptococcus pneumoniae, nontypeable Haemophilus influenzae

SUBMITTER: Kafer LA 

PROVIDER: S-EPMC12889608 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature