Single-Cell Atlas of Transcription and Chromatin States Reveals Regulatory Programs in the Human Brain.

ABSTRACT: Directly measuring chromatin states alongside transcription is essential for understanding how cell-type-specific regulatory programs are established and maintained in the adult human brain. We present a large-scale single-cell multimodal atlas generated by jointly profiling transcriptome with active (H3K27ac) and repressive (H3K27me3) histone modifications across 18 brain regions. We profile >750,000 nuclei spanning 160 cell types and integrate these data with chromatin accessibility, DNA methylation, 3D genome architecture, and spatial transcriptome. This framework annotates >500,000 regulatory elements and resolves cell-type-specific chromatin states. We link enhancers to target genes, infer gene regulatory networks, and classify chromatin interactions, revealing neuron-enriched long-ra

SUBMITTER: Xie Y 

PROVIDER: S-EPMC12889664 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature