Quantification of foscarnet with chromogenic and fluorogenic chemosensors: indicator displacement assays based on metal ion coordination with a catechol ligand moiety.

ABSTRACT: The catechol moiety in a chromophore was used in an indicator displacement assay for the chemosensing of the antiviral drug foscarnet (trisodium phosphonoformate, abbreviated as PFA). Applications of two methods were investigated, namely UV-Vis absorption and fluorescence spectroscopy measuring coordination of a metal to a catechol-based indicator. Yb³⁺ complexation with chromogenic pyrocatechol violet in 10 mM HEPES buffer at pH 7.0 yields a blue chemosensor that responds to the presence of PFA with the release of yellow pyrocatechol violet (PV). The YbPV coordination complex responds linearly to the PFA concentration with a 2 μM detection limit. Metal ion complexation of a range of metal ions (trivalent Al, Ga, In, Sc, La, Gd, Er, Yb, and Fe, and divalent Cu) to the fluorescen

SUBMITTER: Gaidamauskas E 

PROVIDER: S-EPMC12890012 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature