Bioenergetic and immunological characterization of cryopreserved peripheral blood mononuclear cells (PBMCs) isolated from blood and buffy coat.

ABSTRACT:

Background

Mitochondrial dysfunction (MD) and inflammaging are hallmarks of non-communicable diseases and play a pivotal role in the ageing process. Determination of mitochondrial function (MF) in Peripheral Blood Mononuclear Cells (PBMCs) represents a minimally invasive method for assessing the pathophysiological state. However, the necessity to measure the cells in a fresh state is a challenge especially in multicenter investigational studies. Therefore, we investigated the mitochondrial and immunological properties of cryopreserved PBMC isolated from whole blood and buffy coats over a storage period of 6 months to establish a procedure to assess MD in frozen samples.

Methods

PBMCs were isolated from whole blood and buffy coats using gradient density centrifugation and cry

SUBMITTER: Dieter F 

PROVIDER: S-EPMC12894023 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature