Initial characterization of M₂-muscarinic receptor overexpressing mouse heart.

ABSTRACT: There are five muscarinic receptor subtypes expressed in the human heart. The main subtype is the M₂-muscarinic receptor. We hypothesized that overexpression of the M₂-muscarinic receptor should augment any contractile effects that are M₂-muscarinic receptor-mediated. Therefore, we generated a transgenic mouse with overexpression of the human M₂-muscarinic receptor under the control of the heart-specific α-myosin heavy chain promoter (M₂-TG). We performed contraction experiments with electrically stimulated (1 Hz) left atrial preparations (LA) and spontaneously beating right atrial preparations (RA) from adult M₂-TG or from adult wild-type littermate mice (WT). We confirmed the expression of the human M₂-muscarini

SUBMITTER: Gergs U 

PROVIDER: S-EPMC12894120 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature