Integrated transcriptomic and metabolic profiling reveals the molecular mechanism of improved nitrogen metabolism in walnut (<i>Juglans regia</i> L.) roots mediated by soybean intercropping.

ABSTRACT:

Introduction

The practice of intercropping young walnut orchards with soybeans has emerged as a promising approach within the framework of sustainable agricultural management. However, the precise impacts of soybean intercropping on nitrogen metabolism in walnut roots remain insufficiently elucidated.

Methods

In this study, 'Yuanlin' walnut and 'Longhuang 3' soybean were used as experimental materials, with two planting patterns established: walnut monoculture and walnut-soybean intercropping. A combination of metabolomics, transcriptomics (RNA-seq), and physiological index determination was employed to systematically investigate the regulatory mechanisms of nitrogen metabolism in walnuts under the intercropping system.

Results and discussion

The results showed that t

SUBMITTER: Ren J 

PROVIDER: S-EPMC12894250 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature