First report of a urinary <i>Pseudomonas juntendi</i> carrying <i>bla</i> _NDM-1 and <i>bla</i> _IMP-15 co-integrated into the chromosome via <i>ICE-IS91</i> and integron<i>-Tn402</i>-like transposition modules.

ABSTRACT:

Background

Pseudomonas juntendi is an emerging opportunistic pathogen first described in 2019, whose antimicrobial resistance mechanisms and clinical significance remain poorly understood. In this study, we report the first urinary isolate of P. juntendi (PJ1) co-harboring bla _NDM-1 and bla _IMP-15, and comprehensively analyzed its phylogeny, resistance architecture, and biological characteristics.

Methods

Species identification and phylogenetic placement were determined using whole-genome sequencing and average nucleotide identity analyses. Genomic annotation was applied to resolve the structure of resistance islands. Biofilm formation, stress tolerance, and virulence were assessed through crystal violet staining, environment

SUBMITTER: Wang Z 

PROVIDER: S-EPMC12894362 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature