Analysis of Transcriptome and Differentially Expressed Genes in Chicken Primordial Germ Cells.

ABSTRACT: Achieving successful primordial germ cell (PGC)-based genome editing requires a deep understanding of their molecular identity. For the first time, a comparative transcriptomic analysis of chicken PGCs and adult liver cells to define their specific gene expression signature was performed. PGCs were isolated from Rhode Island Red chicken embryos, cultured, and subjected to RNA sequencing alongside liver tissue. Differential expression analysis with Benjamini-Hochberg correction identified 1909 differentially expressed genes (DEGs). Functional annotation revealed that PGCs possess a unique transcriptional landscape, characterized not only by enhanced proliferation and metabolic activity but also by a profound molecular convergence with neural crest cells. This is evidenced by the upregulatio

SUBMITTER: Azovtseva AI 

PROVIDER: S-EPMC12897042 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature