Comprehensive Analysis of Metabolome and Transcriptome Reveals Physiological Processes Related to Larval Development of Barnacles (<i>Megabalanus volcano</i>).

ABSTRACT:

Background

Barnacles are important marine fouling organisms, and their complex life cycle involves key metamorphic nodes from nauplius to cyprid larvae and then to sessile adults. However, the molecular mechanisms underlying their larval development remain poorly understood. Metabolomics and transcriptomics are powerful tools for exploring biological development pathways and regulatory networks.

Methods

We employed non-targeted metabolomics and transcriptomics to analyze three key developmental stages of embryonic stage, nauplius stage, and cyprid stage. Differential metabolites were screened using fold change (FC), p-value, and variable importance in projection (VIP) values, while DEGs were identified with adjusted p-value and |log₂(fold change)| cr

SUBMITTER: Zheng Z 

PROVIDER: S-EPMC12897097 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature