Rhythmic Mechanisms Governing CAM Photosynthesis in <i>Kalanchoe fedtschenkoi</i>: High-Resolution Temporal Transcriptomics.

ABSTRACT: Crassulacean acid metabolism (CAM) is a specialized photosynthetic pathway that enhances water-use efficiency by temporally separating nocturnal CO₂ uptake from daytime decarboxylation and carbon fixation. To uncover the regulatory mechanisms coordinating these temporal dynamics, we generated high-resolution, 48 h time-course transcriptomes for the CAM model Kalanchoe fedtschenkoi under both 12 h/12 h light/dark (LD) cycles and continuous light (LL). A rhythmicity analysis revealed that diel light cues are the dominant driver of transcript oscillations: 16,810 genes (54.3% of annotated genes) exhibited rhythmic expression only under LD, whereas just 399 genes (1.3%) remained rhythmic under LL. A smaller set of 3009 genes (9.7%) oscillated in both conditions, indicating th

SUBMITTER: Hu R 

PROVIDER: S-EPMC12897691 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature