Valproic Acid Stimulates Release of Ca²⁺ from InsP₃-Sensitive Ca²⁺ Stores.

ABSTRACT: Calcium (Ca²⁺)signaling dysfunction is a central contributor to neuronal hyperexcitability and seizure propagation in epilepsy, yet the intracellular mechanisms underlying the actions of valproic acid (VPA) remain incompletely understood. In this study, we investigated whether VPA modulates Ca²⁺ homeostasis at the level of the endoplasmic reticulum (ER) and how this action influences cytosolic Ca²⁺ dynamics associated with epileptiform activity. ER Ca²⁺ levels were directly measured using ER-targeted aequorin in HeLa and PC12 cells, while cytosolic Ca²⁺ signals were monitored by fura-2 fluorescence imaging in bovine chromaffin cells exposed to veratridine, a model of sustained sodium channel activation and Ca²⁺ oscillations.

SUBMITTER: Ruiz-Nuno A 

PROVIDER: S-EPMC12898086 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature