Chemical Profiling, Molecular Docking, and Mechanistic Anticancer Activity of <i>Pinus sylvestris</i> Essential Oil in SH-SY5Y and U-87MG Cells.

ABSTRACT: Pinus sylvestris essential oil (PSEO) has gained increasing interest as a natural anticancer candidate due to its bioactive phytochemical composition and potential to modulate apoptosis-related pathways. In this study, the chemical profile of PSEO was characterized by GC-MS, revealing oxygenated monoterpenes and monoterpene hydrocarbons as dominant constituents. Human brain (U-87MG) and peripheral nervous system (SH-SY5Y) tumor cells were treated with PSEO to evaluate cytotoxicity and mechanistic responses. Cell viability was assessed using the MTT assay, and 24-h IC₅₀ values were determined as 47.93 µg/100 µL for U-87MG and 71.63 µg/100 µL for SH-SY5Y, which were subsequently used for all mechanistic analyses. IC₅₀ exposure significantly increased intracellula

SUBMITTER: Dervisoglu G 

PROVIDER: S-EPMC12898508 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature