Integrating Metabolic and Gene Expression Profiling of Glucosinolate Biosynthesis Under Drought Stress in <i>Brassica oleracea</i>.

ABSTRACT: Drought stress induces pronounced metabolic and transcriptional reprogramming of glucosinolate (GLS) biosynthesis in Brassica oleracea. An integrative approach combining HPLC-based quantification of individual GLSs, quantitative real-time PCR of core biosynthetic and regulatory genes, correlation-based network analysis, and in silico promoter characterization was applied to evaluate drought responses across genetically diverse accessions. Drought triggered strong, accession-specific shifts in GLS composition, with sinigrin content increasing from 35.9% to 55.1% in BR1 and glucoerucin reaching up to 80.2% in CCP1, while indolic GLSs such as glucobrassicin and neoglucobrassicin accounted for >75% of total GLSs in CV2 and CCP3. Hierarchical clustering separated accessions into four dis

SUBMITTER: Ben Ammar H 

PROVIDER: S-EPMC12898713 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature