Rapid Authentication of Flowers of <i>Panax ginseng</i> and <i>Panax notoginseng</i> Using High-Resolution Melting (HRM) Analysis.

ABSTRACT: The flowers of Panax ginseng C. A. Mey. (PG) and Panax notoginseng (Burkill) F. H. Chen ex C. H. Chow (PN) are morphologically indistinguishable after drying, leading to prevalent adulteration that compromises product quality and consumer safety. To address this issue, we developed a rapid, closed-tube molecular authentication method based on high-resolution melting (HRM) analysis. Species-specific primer pairs were designed to target the conserved ITS and rbcL-accD regions, with PNG-2 selected as the optimal candidate owing to its stable genotyping performance and moderate GC content. Our results established GC content, rather than amplicon length, as the primary determinant of the melting temperature (Tm). Notably, the experimentally measured Tm

SUBMITTER: Wang M 

PROVIDER: S-EPMC12898732 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature