Efficient Degradation of Monoacylglycerols by an Engineered <i>Aspergillus oryzae</i> Lipase: Synergistic Effects of sfGFP Fusion and Rational Design.

ABSTRACT: Monoacylglycerols (MAGs) are significant intermediate byproducts in the hydrolysis of oils and fats. The accumulation of MAGs not only reduces the quality and purity of the final products in biodiesel production and edible oil refining but also poses challenges for downstream separation processes. Therefore, the development of efficient biocatalysts for the specific MAG conversion is of great industrial importance. The lipase from Aspergillus oryzae (AOL) has shown potential for lipid modification; however, the wild-type enzyme (WT) suffers from poor solubility, tendency to aggregate, and low specific activity towards MAGs in aqueous systems, which severely restricts its practical application. In this study, a combinatorial protein engineering strategy was employed to overcome these

SUBMITTER: Wang Y 

PROVIDER: S-EPMC12899640 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature