Enhanced Antiplatelet Activity of Nitrated Fatty Acid Extracts from <i>Phaseolus vulgaris</i> L.

ABSTRACT: Dietary bioactive compounds are increasingly explored as complementary cardioprotective strategies, and the nitration of unsaturated fatty acids has emerged as a process capable of enhancing antiplatelet properties. This study investigated whether Phaseolus vulgaris L. extracts can generate nitrated fatty acids under gastric-like conditions and evaluated their effects on human platelet function. Bean extracts and major fatty acids were nitrated in vitro and tested using washed platelets to assess cytotoxicity, TRAP-6 and collagen-induced aggregation, activation markers (P-selectin, CD63), and mitochondrial responses including membrane potential, ROS production, and Ca²⁺ dynamics. Nitrated extracts markedly inhibited TRAP-6 induced aggregation (IC₅₀ ≈ 1.8

SUBMITTER: Rodriguez L 

PROVIDER: S-EPMC12899701 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature