Characterization of Extracellular Vesicles from Cell Suspension Cultures of <i>Coffea arabica</i> L.

ABSTRACT: A protocol was developed for the isolation and characterization of extracellular vesicles (EVs) from Coffea arabica cell suspension cultures (CSCs). The isolation method involved differential ultracentrifugation of the CSC filtrate, yielding two fractions: the pellet after 100,000×g for 36 min (100k×g) and the pellet obtained from the previous supernatant after 125,000 g for 6 h (125k×g). Both fractions were characterized by size, morphology, and proteomic profiles (ProteomeXchange identifier PXD071909). While no significant differences in average EV size were observed between the two fractions, proteomic analysis revealed distinct quantitative and compositional variations. The 100k×g fraction was enriched in proteins associated with cell periphery

SUBMITTER: Bonaventura AD 

PROVIDER: S-EPMC12899826 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature