Genome-Driven Discovery of Anti-MDR Bacterial Heptapeptides from a Cold-Seep-Derived <i>Bacillus</i> Strain.

ABSTRACT: With the increasing emergence of multidrug-resistant (MDR) bacteria, there is an urgent need to discover new antibiotics. In this study, genome mining coupled with anti-bacterial assay guided the targeted isolation of two new heptapeptides nobilamide Q3 (1) and R3 (2). These compounds were identified as new stereoisomers of the known scaffold A-3302-B (3). The structures of these compounds were elucidated through a combination of MS, NMR spectroscopy and Marfey's analysis. Anti-MDR bacterial assays showed that compounds 2 and 3 exhibited effective growth inhibition against the Gram-positive MDR bacterial strain Staphylococcus aureus CCARM 3090 with MIC values of 3.25-6.5 μg/mL. Notably, our study reveals stereochemistry-dependent differences in the

SUBMITTER: Li H 

PROVIDER: S-EPMC12899971 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature