A method to unravel complexity in human peripheral blood B-cell subsets through multiomic cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq) analyses.

ABSTRACT: Classifying heterogeneous human B cells into discrete subsets based on surface phenotype alone remains a major challenge. We discovered a mouse splenic IgM+IgDlow/- B-cell subset (BDL) that induces proliferation of CD4+Foxp3+ T regulatory cells, making them a therapeutic target for autoimmunity. Because they are rare and lack sufficient cell surface markers, a multiomics strategy is required to identify and track them in humans. The lack of correlation between mRNA expression levels and cell surface expression is a drawback to a transcriptome-only single-cell RNA sequencing (scRNA-seq) approach because "novel" cell populations identified by surface protein transcripts may prove impossible to validate. To that end, a method was developed using human peripheral blood (PB) IgM+IgDlow/- B cell

SUBMITTER: Meinhardt NJ 

PROVIDER: S-EPMC12900538 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature