Analysis of salt resistance conferred by salt overly sensitive 3 protein from mulberry (<i>Morus notabilis</i>).

ABSTRACT:

Introduction

Soil salinization severely threatens crop productivity and agricultural sustainability. Mulberry (Morus notabilis) is an economically important woody crop, but the molecular mechanisms of its salt tolerance remain unclear. The conserved Salt Overly Sensitive 3 (SOS3) gene regulates ion homeostasis under salt stress, making it a key target for exploring mulberry's salt adaptation.

Methods

We cloned MnSOS3 from M. notabilis, constructed a prokaryotic expression vector (pCold-TF-MnSOS3) for transformation into Escherichia coli BL21, and generated MnSOS3-overexpressing transgenic tobacco (Nicotiana benthamiana) via Agrobacterium-mediated transformation. Salt tolerance was evaluated in E. coli (0.6

SUBMITTER: Liu D 

PROVIDER: S-EPMC12900689 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature