Osteoclasts affect the anti-cancer activity of NK cells.

ABSTRACT:

Introduction

Non-small cell lung cancer (NSLSC) stem cells (CSCs) have been shown to be responsible for bone metastasis by interacting with osteoclasts (OCs) and creating an immunosuppressive environment in the bone pre-metastatic niche. Now, we investigated the interaction among OCs, IL-15-stimulated NK cells and CSCs to understand whether NK cells can interfere with the pro-metastatic crosstalk between OCs and CSCs.

Methods

In vitro co-cultures of autologous OCs, NK cells and spheres enriched for CSC from NSCLC A549 cell line (A549/s) were set up both on plastic and bone slices, and OC activity was evaluated through quantification of tartrate-resistant acid phosphatase and of resorption area. The expression of NK cell receptors and ligands was studied through flow c

SUBMITTER: Orrico C 

PROVIDER: S-EPMC12901471 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature