Total RNA and MicroRNA Transcriptomic Responses of Human Retinal Muller Glial Cells to Infection with <i>Toxoplasma gondii</i> Tachyzoites.

ABSTRACT:

Purpose

Ocular toxoplasmosis, caused by infection with Toxoplasma gondii, is characterized by retinal necrosis and reactive intraocular inflammation. Müller glial cells are a principal retinal host cell population for T. gondii. The goal of this research was to delineate potential involvements of Müller glial cells in ocular toxoplasmosis at a molecular level.

Design

Laboratory-based study.

Samples

Human retinal Müller glial cells infected with T. gondii plus noninfected cells.

Methods

Monolayers of Müller glial cells isolated from human retina (6 donor eye pairs) were infected for 24 hours with GT1 or GPHT strain T. gondii tachyzoites (multiplicity of infection of 5), or incubated in parallel without infection. Total RNA and small R

SUBMITTER: Rochet E 

PROVIDER: S-EPMC12902236 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature