PUM2 knockdown regulates the expression and alternative splicing of genes associated with myocardial fibrosis in H9C2 cells.

ABSTRACT: Myocardial fibrosis (MF) represents a major pathological alteration observed in the progression of numerous heart conditions. The presence of excessive MF is key in the progression of numerous heart diseases, which may lead to heart failure. Abnormal expression of pumilio RNA-binding family member 2 (PUM2), an RNA-binding protein, is a contributing factor in the development of a variety of diseases. The present study aimed to investigate how PUM2 affected the expression and alternative splicing of genes associated with MF in H9C2 cells, thus evaluating the role of PUM2 in this context. Inhibition of PUM2 expression in H9C2 cardiomyocytes was achieved through small interfering RNA transfection. Cell viability was assessed using a Cell Counting Kit-8 assay, while apoptosis was detected throu

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC12902810 | biostudies-literature | 2026 Apr

REPOSITORIES: biostudies-literature