Optimization of Bioactive Polysaccharide Extraction from <i>Protaetia brevitarsis seulensis</i> Larvae Using Deep Eutectic Solvents.

ABSTRACT: An optimized deep eutectic solvent (DES) method was developed to enhance polysaccharide extraction from Protaetia brevitarsis seulensis larvae (PBSL) compared to conventional water extraction (CWE). Among five choline chloride-based DESs, 40% aqueous choline chloride:glycerol (1:2) was optimal. The response surface methodology with a Box-Behnken design yielded precise conditions (78.1 °C, 1.6 h, and 1:21 g/g), achieving 18% higher yield and 38% greater purity than CWE. Both extracts were identified as acidic heteroglycans with similar monomeric profiles, but DES polysaccharides showed lower molecular weight, higher branching, improved morphology, and better stability. DES-extracted polysaccharides exhibited stronger antioxidant activity and more potent anti-inflammatory effects in l

SUBMITTER: Ojulari OV 

PROVIDER: S-EPMC12903018 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature