Differential synaptic signaling responses in human cortical organoids after photon and proton irradiation.

ABSTRACT: Brain tumors are the most common solid cancer in children, with radiotherapy being a primary treatment. Proton therapy, with its precise dose distribution, is increasingly being used in these patients to minimize damage to the developing brain. However, the biological effects of proton irradiation on the human brain remain unclear. To investigate this, human cortical organoids were exposed to conventional photons, plateau protons, and spread-out Bragg peak (SOBP) protons, followed by comparative transcriptomic profiling. While photons and protons induced similar transcriptional profiles characterized by apoptosis and downregulation of DNA replication, SOBP protons uniquely downregulated genes involved in brain development and synaptic signaling. Functional calcium imaging, cell deconvoluti

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC12903084 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature