Next Generation Sequencing of Tracheal Aspirates in Children With Tracheostomy A Prospective Case-Control Study.

ABSTRACT:

Background

Tracheostomies are increasingly performed in children. The treatment of infections in tracheostomized patients is challenging because of the distinction between chronic colonization and acute infection. Next-generation sequencing (NGS) is a promising alternative for identifying and quantifying pathogens in a short period. This study investigated the utility of NGS of tracheal aspirates from healthy and tracheostomized children (TC).

Methods

This monocentric prospective case-control study recruited children with long-term TC and compared the colonization of the lower airways with a control group of children who had undergone elective general surgery. Tracheal aspirates were analyzed using three methods: bacterial culture, polymerase chain reaction (PCR), and NGS.

SUBMITTER: Brensing P 

PROVIDER: S-EPMC12903189 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature