Antimicrobial effects of Bacillus safensis BS05 cell free supernatant against Campylobacter jejuni and its probiotic evaluation.

ABSTRACT: Campylobacter jejuni (C. jejuni) is a significant foodborne pathogen. The rise of antibiotic-resistant strains has diminished the effectiveness of conventional treatments, highlighting the urgent need for safe and effective alternatives. In this study, a Bacillus safensis (B. safensis) strain (BS05) was isolated from goats and assessed for its probiotic properties and antagonistic activity against C. jejuni. BS05 formed rough colonies on agar, and scanning electron microscopy revealed predominantly short rod-shaped cells. The cell-free culture supernatant (CFS) of BS05 inhibited multiple C. jejuni strains of diverse sequence types. This inhibition was evidenced by reduced optical density (OD₆₀₀) values and MIC-like growth inhibition assays, indicating a dose-dependent antagonist

SUBMITTER: Zhang X 

PROVIDER: S-EPMC12903218 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature