The OsWRKY47-OsMYB30/OsWRKY39 module confers both physical and chemical defenses against Magnaporthe oryzae to enhance rice blast resistance.

ABSTRACT: The infection cycle of Magnaporthe oryzae (M. oryzae) in rice typically involves initial invasion through penetration of the leaf epidermis, followed by expansion into neighboring cells. However, few studies have identified single genes that mediate defense against both invasion and expansion. In this study, we demonstrate that OsWRKY47 positively regulates resistance to both stages of M. oryzae infection. Mechanistic analyses indicate that OsWRKY47 transcriptionally activates OsMYB30 during the early stage, thereby enhancing lignin accumulation and strengthening physical barriers against fungal invasion. At later stages, OsWRKY47 represses OsWRKY39 expression, thereby inhibiting M. oryzae expansion. Knockout of OsWRKY39 leads to increased accumulation of stevioside, a metabolite that acti

SUBMITTER: Liu Y 

PROVIDER: S-EPMC12903394 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature