The transcription factor ANAC017 links mitochondrial retrograde signaling with the ubiquitin-proteasome system to control mitochondrial function in Arabidopsis.

ABSTRACT: Mitochondrial biogenesis requires the import of more than a thousand proteins encoded by nuclear DNA. The translocase of the outer mitochondrial membrane (TOM) complex serves as the primary gateway for specific recognition of precursor proteins, which are synthesized in the cytosol. Little is known about the regulation of the abundance of the TOM complex. Using forward genetics, we identified key 26S proteasome subunits, including REGULATORY PARTICLE NON-ATPASE1A (RPN1A), that affect the abundance of TOM-complex subunits through the ubiquitin-proteasome pathway. Loss of proteasome function through rpn1a mutation or MG132 treatment increased the abundance of TOM20 isoforms and induced mitochondrial stress marker genes. By contrast, overexpression of ANAC017, an endoplasmic reticulum-anchore

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC12903413 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature