The C-terminal SUMOylation-dependent regulation of αKNL2 governs its centromere targeting and interaction with CENH3.

ABSTRACT: The centromere is a specialized domain that facilitates chromosome segregation during mitosis and serves as the site of kinetochore formation. KINETOCHORE NULL2 (αKNL2) is essential for the recognition and loading of the centromeric histone H3 variant CENH3 at centromeres. A yeast two-hybrid screen for αKNL2 interactors identified components of the SUMOylation pathway. However, the role of αKNL2 SUMOylation in Arabidopsis has not yet been determined. In this study, we demonstrated that the C-terminal region of αKNL2 (designated αKNL2-C) interacts with small ubiquitin-like modifier 3 (SUMO3) and ULP1d, as shown by bimolecular fluorescence complementation and co-immunoprecipitation assays. Bioinformatic and functional analyses of αKNL2-C identified three SUMOylation sites and two SUMO-intera

SUBMITTER: Kalidass M 

PROVIDER: S-EPMC12903445 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature