A single nucleotide mutation of <i>BnaC05.POLIB</i> creates yellow-white chimeric flower in <i>Brassica napus</i>.

ABSTRACT: Flower color is a key trait influencing insect pollination and ornamental value, yet the molecular mechanisms underlying heterozygous flower color remain unclear. In this study, we identified the creation of a yellow-white chimeric flower (cf) mutation in Brassica napus, characterized as the coexistence of yellow and white colors on petals of the same flower. Genetic analysis revealed that chimeric flower formation is controlled by a completely dominant gene. Map-based cloning, transgenic complementation, and CRISPR/Cas9 experiments consistently confirmed that BnaC05G0385300ZS on chromosome C05 is the causal gene of CF, which encodes a plastid DNA polymerase IB (BnaC05.POLIB). A G-to-A mutation in the seventh exon results in a D742N substitution, which disrupts

SUBMITTER: Xia R 

PROVIDER: S-EPMC12903450 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature