PHR1-like 7 and phosphatidic acid oppositely regulate TAG degradation and seed oil accumulation in Arabidopsis.

ABSTRACT: Triacylglycerol (TAG) degradation plays an important role in seed oil accumulation, but how this catabolic process is transcriptionally regulated in developing seeds remains poorly understood. Here, we identify a MYB-like helix-turn-helix (HTH) transcriptional regulator, PHR1-like 7 (PHL7), that enhances TAG degradation and decreases seed oil accumulation in Arabidopsis thaliana. PHL7 knockout (KO) plants (phl7) exhibited an approximately 10% increase in seed oil content, whereas PHL7 overexpression (OE) decreased oil content by 8%-13%. Compared with wild-type plants, phl7 displayed reduced expression of TAG degradation genes, including SUGAR-DEPENDENT 1 (SDP1), whereas OE lines exhibited elevated expression. Chromatin immunoprecipitation, electrophoretic mobility shift assays, and transac

SUBMITTER: Tang S 

PROVIDER: S-EPMC12903457 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature