Cryopreservation of Avian Erythrocytes Using Macromolecular Cryoprotectants.

ABSTRACT: Erythrocyte (red blood cell) cryopreservation is an essential biotechnology for healthcare (transfusions), diagnostics, conservation, and fundamental research. Mature mammalian erythrocytes are anucleate, do not divide, and cannot undertake protein biosynthesis. In contrast, avian erythrocytes have a distinct evolutionary pathway, retaining their nucleus, and are capable of other limited functions, such as protein biosynthesis, but have a shorter shelf life than mammalian erythrocytes. Here, we report the successful cryopreservation of avian red blood cells using a polyampholyte (macromolecular cryoprotectant), which can reduce intracellular ice formation. Under optimized conditions with 10% DMSO and 60 mg·mL^-1 of polyampholyte, ∼90% recovery could be achieved, outperforming a c

SUBMITTER: Bissoyi A 

PROVIDER: S-EPMC12903467 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature