Synergistic modulation of ABC transporter-mediated multidrug resistance by <i>Kalanchoe laciniata</i> (L.) DC. phytochemicals: integrative phytochemical profiling, network pharmacology, and molecular docking insights.

ABSTRACT: Kalanchoe laciniata (L.) DC. is an underexplored medicinal plant with promising anticancer potential. This study evaluated the phytochemical profile, antioxidant activity, and multidrug resistance (MDR) modulating effects of its methanolic extract (KLM), with emphasis on ABC transporter regulation. Soxhlet methanol extraction yielded the highest phenolic and tannin contents and exhibited strong antioxidant activity. GC-MS and LC-MS analyses identified bioactive flavonoids and phytosterols, including quercetin and kaempferol derivatives. Network pharmacology revealed key interactions with ABC transporters and hub genes such as AKT1 and TP53. Molecular docking and dynamics simulations demonstrated stable binding of KLM-derived flavonoids with BCRP, MRP1, and AKT1, supporting their rol

SUBMITTER: S AR 

PROVIDER: S-EPMC12903875 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature