Investigating the Trans Effects of IS<italic>1</italic> Transposases on Intragenomic DNA Movements in <italic>Escherichia coli</italic>.

ABSTRACT:

Introduction

The transposase protein, InsAB', is the key cytoplastic determinant that catalyzes IS1 movement within a single DNA molecule or between two DNA molecules. Regulation of InsAB' synthesis, its structural characteristics, and its potent cis effect on IS1 transposition have been subjects of thorough investigation. However, limited efforts have been devoted to measuring the levels of IS1 transcripts and transposases made by native IS1 elements and examining their trans-acting function, particularly concerning their role in transposing otherwise immobile IS1 elements and other target DNA segments within the bacterial genome.

Methods

All constructs were made on the Escherichia coli chromosome using the lambda-Red system. IS1 transposition frequencies were determined us

SUBMITTER: Ord JW 

PROVIDER: S-EPMC12903888 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature