Rapid drug resistance prediction in positive <i>Mycobacterium tuberculosis</i> clinical samples using an extensive targeted next-generation sequencing panel.

ABSTRACT: Tuberculosis (TB) remains a global health challenge, exacerbated by the emergence of drug-resistant Mycobacterium tuberculosis strains. Most methods for drug susceptibility testing (DST) are culture-dependent and time consuming, possibly delaying optimal TB-treatment. This study aimed to develop an extensive targeted next-generation sequencing (tNGS) approach for rapid genotypic DST directly from clinical samples. We designed a tNGS panel comprising 30 amplicons targeting 19 genomic regions associated with resistance to 20 antibiotics. This method was applied to 71 smear-positive (0-3+) pulmonary TB clinical samples collected at the Portuguese National Reference Laboratory. DNA was extracted and amplified using multiplex PCRs, followed by sequencing on Oxford Nanopore Technologies M

SUBMITTER: Rosendal E 

PROVIDER: S-EPMC12903939 | biostudies-literature | 2026 Dec

REPOSITORIES: biostudies-literature