Autopolyploidization-Induced Chromatin Remodeling Regulates Leaf Size Variation in Brassica rapa.

ABSTRACT: Whole-genome duplication is a key evolutionary mechanism influencing gene regulation and trait development; however, how successive genome duplications reshape chromatin at the genome-wide scale and thereby drive phenotypic innovation remains unclear. To dissect the effects of genome doubling on chromatin dynamics, gene expression, and associated trait differences, monoploid, diploid, and autotetraploid Brassica rapa L. ssp. pekinensis lines are generated with an identical genomic background and performed integrative analyses using ATAC-seq, ChIP-seq (H3K4me3, H3K27ac, H3K27me3), and RNA-seq. By establishing this uniform ploidy series, nonlinear and stage-specific chromatin and transcriptional reprogramming during autopolyploidization are revealed. Increased ploidy reprogrammed chromatin a

SUBMITTER: Dong H 

PROVIDER: S-EPMC12904041 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature