Label-Free Leukocyte Biophysical Profiling Using Impedance-Deformability Cytometry for Rapid Cardiovascular Risk Stratification.

ABSTRACT: Type 2 diabetes mellitus (T2DM) presents a global health burden with cardiovascular disease (CVD) as its leading cause of mortality. A rapid clinical-adaptable microfluidic workflow for label-free CVD risk profiling based on neutrophil biophysical abnormalities is developed. This high-throughput single cell (>1,000 cells min^-1) "electro-mechano-phenotyping" method integrates Uniform Manifold Approximation and Projection analysis to assess leukocyte biophysical changes (size, deformability, and impedance properties) linked to inflammation, thrombosis, and hyperglycemia in vitro, and in diabetic and diabetic atherosclerosis-prone mouse models. In a clinical study of healthy, pre-diabetes, diabetes, and diabetic patients with CVD (DM-CVD) subjects (n = 10-11 per group), DM-CVD neut

SUBMITTER: He L 

PROVIDER: S-EPMC12904061 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature