A TtAgo-Driven Autocatalytic Circuit with Thermal-Enhanced Kinetics for One-Pot Nucleic Acid Detection.

ABSTRACT: Catalytic DNA circuits hold significant promise for nucleic-acid-based diagnostics, yet they remain hindered by slow reaction kinetics and the non-universal nature of one-pot approaches. Here, a universal catalytic DNA circuits termed TACTIC (Thermus thermophilus Argonaute (TtAgo) protein-driven autocatalytic circuit) is developed for one-pot detection of DNA and RNA in multiple clinical samples. TACTIC employs the heat-activated cleavage activity of TtAgo to accelerate reaction kinetics of rolling circle amplification (RCA) by producing an efficient circular template with Gibbs free energy approaching zero at 70. Combined with TtAgo cleavage-mediated explosive regeneration and accumulation of target mimics, an autocatalytic positive-feedback circuit is successfully constructed for univers

SUBMITTER: Xie Z 

PROVIDER: S-EPMC12904063 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature