A Membrane Fluidization Strategy Significantly Boosts Photodynamic Therapy and Antitumor Immunity of Porphyrin-Lipid Nanoparticles.

ABSTRACT: Porphysomes (PS) are self-assembled porphyrin-lipid nanoparticles with intrinsic multifunctionality for cancer theranostics. We recently discovered that incorporating EDTA-conjugated lipids (EDTA-lipids) into lipid nanoparticle formulations significantly enhances intracellular uptake by modulating the cell membrane fluidity. To retain the favorable in vivo properties of PS while improving intracellular porphyrin delivery for effective photodynamic therapy (PDT), we developed a dual cellular- and vascular-enriched porphysome (DE-PS) containing 30 mol % cholesterol, 5 mol % PEG2000-DSPE, 27 mol % pyro-lipid, and 38 mol % EDTA-lipid. DE-PS demonstrated favorable pharmacokinetics (t _1/2β = 7.5 h), robust photostability (>99% fluorescence quenching

SUBMITTER: Ho T 

PROVIDER: S-EPMC12904080 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature